Absolute Precision. From Single Proteins to Complex Interactions. 

The PICO Binding Reaction

No complex washing steps, no surface immobilization. Just mix your purified sample with the labeled antibodies and let the molecular proximity do the rest.

• Solution-Based Binding: The entire reaction happens completely in solution. Your targets maintain their native conformation, ensuring realistic, unhindered biological interactions.
• No Surface Loss: Because there are no washing steps or solid-phase binding, you completely eliminate the background noise and sample loss typical of traditional assays.
• Intact Membrane Integrity: For EV analysis, the entire binding reaction is completely detergent-free. Your vesicles remain 100% intact from the initial mix to the final readout.

Digital PCR
Partitioning & Multiplex Amplification

This is where the biochemistry turns into digital data. The sample mixture is loaded directly into the digital PCR system, whether it utilizes microfluidic nanoplates, chips, or droplet generation.

• Micro-Partitioning: The sample is instantly distributed across thousands of isolated nanoliter cavities or oil droplets. Individual targets and their bound DNA-labeled antibodies are locked into separate, independent micro-reactions.
• Proximity-Driven Amplification: Once partitioned, the standard dPCR master mix amplifies the DNA barcodes. A distinct fluorescent signal is generated only if the specific target markers are present together in that individual partition.
• Multiplexing Opportunities: By using the multiple fluorescent dye channels on your dPCR hardware, you can simultaneously detect different target combinations, subpopulation markers, or distinct protein complexes in a single run.
• Direct Molecular Counting: Because dPCR operates on a binary (positive/negative) readout per partition, the system counts the absolute number of fluorescent events. No reference standards or standard curves required.

Automated Data Analysis with PIQuant® Software

Once your digital PCR run is complete, the raw data is seamlessly converted into clear, actionable results without manual calculation or complex spreadsheets.

• Effortless Upload: Simply upload the raw data files directly from your dPCR instrument into the intuitive PIQuant® software interface.
• Automated Partition Counting: PIQuant® instantly scans and analyzes each cavity, compartment, or droplet, identifying the exact ratio of positive-to-negative fluorescent signals.
• Patented Quantification Algorithm: The software utilizes a unique, patented algorithm to filter and calculate the absolute molecular concentration of your target complexes.
• Zero Calibration Needed: Because the algorithm provides direct, absolute quantification, you get precise molecular counts without ever needing to run a standard reference curve.