The Protein Interaction Coupling (PICO) technology enables the detection of proteins, protein interactions, and post-translational modifications with ultra-high sensitivity, zero background, and high parallelism! Together with QIAGENs QIAcuity Digital PCR System, PICO combines the advantages and flexibility of immunoassays, with the sensitivity and robustness of the digital PCR. Watch our technology explainer video to learn more about PICO.
The PICO Protein Detection Trial Kit and 2 additional PICO Labels & Probes are now available in the Actome webshop.
Do you want to try out PICO and experience the unique features of the technology?
With the PICO Protein Detection Trial Kit you will:
• Get acquainted with the PICO workflow
• Set up a PICO assay and perform the dPCR reaction
• Carry out the data analysis with AMULATOR, our web-based analysis software
• Perform relative quantification of your data
• Experience the unique features of PICO, such as ultra-high sensitivity, no background, and wash-free workflow
From now on, two additional PICO Labels & Probes (N6, O7) are available, increasing the flexibility and the scope of PICO! With 4 PICO Labels, two targets can be measured simultaneously in one reaction... in parallel! The additional PICO Probes and Labels are fully compatible with the already available PICO Kits.
... two different proteins simultaneously. Similar to 2-color PICO assays, two antibodies per target are required.
... two different protein interactions simultaneously. One pair of antibodies per protein interaction is required.
... one protein interaction and a single protein simultaneously. One antibody pair is required for the protein interaction and two antibodies are required for the single protein.
... a protein interaction in combination with
measuring the protein level of one of the interacting
partners. Altogether three antibodies are required (one against one of the
partner and two against the other partner).
... a post-translational modification together with measuring the
amount of the modified protein (termed ‘triangular measurement’). Three antibodies are required (two against the protein and one against the modification).