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PICO Technology Overview

Step1 - Labeling

PICO Labeling Service:
We Do the Work, You Get the Data

Don't waste time on manual conjugation. Name your targets, and our scientists will handle the entire process from procurement to strict quality control.

  • Hands-Off Convenience: Simply ship your antibodies to our lab, or let us order them directly from your preferred supplier.
  • Tailored to Your Assay: Choose the exact labels your specific multiplex assay requires.
  • 100% Site-Directed Precision: We guarantee flawless, reproducible coupling targeting non-variable regions—leaving your antigen-binding sites completely untouched.
  • Exact Stoichiometry: Our standardized process ensures absolute molecular consistency, loading exactly 2 labels per heavy chain.
  • Built-In Quality Control: Every batch undergoes rigorous testing to verify the final concentration and absolute labeling efficiency.

    Requirement: This premium service is optimized for IgG antibodies.

The PICO Binding Reaction

No complex washing steps, no surface immobilization. Just mix your purified sample with the labeled antibodies and let the molecular proximity do the rest.

  • Solution-Based Binding: The entire reaction happens completely in solution. Your targets maintain their native conformation, ensuring realistic, unhindered biological interactions.
  • No Surface Loss: Because there are no washing steps or solid-phase binding, you completely eliminate the background noise and sample loss typical of traditional assays.
  • Intact Membrane Integrity: For EV analysis, the entire binding reaction is completely detergent-free. Your vesicles remain 100% intact from the initial mix to the final readout.
Step2 - Binding
Step3 - Amplification

Digital PCR
Partitioning & Multiplex Amplification

This is where the biochemistry turns into digital data. The sample mixture is loaded directly into the digital PCR system, whether it utilizes microfluidic nanoplates, chips, or droplet generation.

  • Micro-Partitioning: The sample is instantly distributed across thousands of isolated nanoliter cavities or oil droplets. Individual targets and their bound DNA-labeled antibodies are locked into separate, independent micro-reactions.
  • Proximity-Driven Amplification: Once partitioned, the standard dPCR master mix amplifies the DNA barcodes. A distinct fluorescent signal is generated only if the specific target markers are present together in that individual partition.
  • Multiplexing Opportunities: By using the multiple fluorescent dye channels on your dPCR hardware, you can simultaneously detect different target combinations, subpopulation markers, or distinct protein complexes in a single run.
  • Direct Molecular Counting: Because dPCR operates on a binary (positive/negative) readout per partition, the system counts the absolute number of fluorescent events. No reference standards or standard curves required.

Automated Data Analysis with PIQuant® Software

Once your digital PCR run is complete, the raw data is seamlessly converted into clear, actionable results without manual calculation or complex spreadsheets.

  • Effortless Upload: Simply upload the raw data files directly from your dPCR instrument into the intuitive PIQuant® software interface.
  • Automated Partition Counting: PIQuant® instantly scans and analyzes each cavity, compartment, or droplet, identifying the exact ratio of positive-to-negative fluorescent signals.
  • Patented Quantification Algorithm: The software utilizes a unique, patented algorithm to filter and calculate the absolute molecular concentration of your target complexes.
  • Zero Calibration Needed: Because the algorithm provides direct, absolute quantification, you get precise molecular counts without ever needing to run a standard reference curve.
Step4 - Analysis

Next-Gen EV Analysis: Beyond the Limits of Size

Using PICO shifts the focus from physical particle size to molecular surface markers, allowing you to see what traditional optical methods miss.

  • Size-Independent Quantification: Detect and count small vesicles (<100 nm) just as reliably as larger ones—bypassing the physical limits of NTA and flow cytometry.
  • Subpopulation Profiling: Identify and isolate specific vesicle populations based on surface marker composition (e.g., tumor-specific markers like HER2+).
  • True Co-Occurrence Analysis: Prove that multiple markers are physically present on the same individual vesicle membrane, rather than just co-existing in bulk solution.
  • 1 µL Sample Conservation: Achieve 100x higher sensitivity than ELISA, extracting maximum digital data from precious, low-volume purified samples.

Stop guessing by size. Start counting by biology. PICO makes it count!


Protein, Protein-Protein interaction detection

The PICO technology redefines how you detect and measure protein-protein interactions (PPIs) and low-abundance proteins, replacing messy bulk assays with digital precision.

  • True Dimer & Complex Detection: Quantify physical protein-protein interactions directly in solution, proving two targets are bound as a complex rather than just co-existing.
  • 200x Higher Sensitivity than Co-IP: Capture transient or weak interactions and ultra-low abundance proteins that are completely invisible to Western Blots or traditional Co-IP.
  • No Surface Matrix Bias: The entire assay takes place completely in solution. Eliminate the background noise, washing artifacts, and sample loss caused by solid-phase binding.
  • Ultra-Low Sample Input: Map complex protein networks using just a fraction of a single cell lysate microliter, conserving precious clinical materials.

Stop masking interactions. Start counting complexes. PICO makes it count!

Site-Specific Antibody Labeling Service

Our premium PICO Custom Labeling Service handles the entire conjugation process for you, turning your standard IgG antibodies into high-performance digital detection units.

  • Hands-Off Convenience: Simply ship your antibodies to our lab, or let us order them directly from your preferred supplier.
  • 100% Site-Directed Precision: We guarantee reproducible coupling targeting only non-variable regions, leaving your critical antigen-binding sites completely untouched.
  • Exact Stoichiometry: Our standardized molecular process ensures absolute consistency, loading exactly 2 DNA labels per heavy chain.
  • Guaranteed Quality Control: Every batch undergoes rigorous testing to verify the precise final concentration and absolute labeling efficiency before it returns to your lab.

You ship the binders, we deliver digital precision. PICO makes it count!